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KMID : 0350519960490020563
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Journal of Catholic Medical College
1996 Volume.49 No. 2 p.563 ~ p.572
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Aberration of Fibroblast Functions Caused by Conditioned Medium of Silicone-sensitized Mouse Splenocytes
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Abstract
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Fibrous capsular contracture has been considered as a major side effect of breast implant using silicone polymer. The etiology of fibrous capsular contracture is not fully determined yet. In the present study, we tried to determine the possible
effect
of immune system on function of fibroblasts which have been known as a major contributing factor in fibrous capsular contracture formation.
I designed three experimental groups using mouse fibroblasts (NIH 3T3), mouse (ICR) lymphocytes and silicone gel. For the preparation of two kinds of conditioned medium of lymphocytes, mouse (ICR) splenocytes were prepared and cultured for one
day.
Silicone conditioned medium (SCM) and silicone free conditioned medium (NCM), were prepared from mouse splenocytes suspension cultured on silicone gel coated surface and naked surface, respectively. I used mouse fibroblasts culture with standard
fresh
medium (FM) as a normal reference. Each conditioned medium of 25% concentration was added onto mouse fibroblasts culture medium. On the second day after inoculation, number of cells, thymidine and proline incorporation of fibroblasts of each
medium
were
measured.
@ES The results were as follows;
@EN 1) The number of fibroblasts showed a significant increase in NCM compared with FM and SCM (P<0.01), but there was no significant difference in number of cells between SCM and FM.
2) The rate of DNA synthesis of fibroblasts showed a significant increase in SCM compared with FM(P<0.001).
3) The rate of collagen synthesis of fibroblasts showed a significant increase in SCM compared with FM (P<0.001) and NCM (P<0.001).
4) The functional activities of DNA and collagen synthesis mediated by same number of fibroblasts were calculated to be increased in SCM compared with NCM and FM (P<0.001).
In conclusion, fibroblasts cultured in SCM had a higher potential to synthesize macromolecules such as collagen and DNA. We can postulate that SCM might have certain amount of unidentified growth stimulant produced by silicone gel sensitized
mouse
splenocytes.
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